BMEN8421

Understanding light microscopy and the interaction of light with biological materials is widely applicable to numerous research programs. In fact, it is a fundamental approach to addressing critical questions at the cellular and subcellular scales. This course will emphasize the fundamentals of light microscopy and microscopes, fundamentals of fluorescence and fluorescence microscopy (transitions, quantum yield, bleaching, lifetime etc.) and practical applications of fluorescence microscopy (confocal microscopy for optical sectioning, multiphoton microscopy, harmonic generation, FRET, FRAP, and fluorescence lifetime in the time and frequency domains). Course material will span theory, practical applications of microscopy and published literature.

prereq: Graduate students in physical sciences (engineering, physics, chemistry etc.), or graduate students with an undergraduate degree in the physical sciences or mathematics, or consent of instructor. In addition to previous course work in engineering and/or physics, a working understanding of microscopy is recommended. Although not required, concurrent or previous enrollment in BMEn 5421 (Biomedical Optics) is recommended.

000356